Endothelial Colony-Forming Cells from Idiopathic Pulmonary Fibrosis Patients Have a High Procoagulant Potential.
Paul BilloirAdeline BlandinièresNicolas GendronRichard ChocronSven GuntherAurélien PhilippeCoralie L GuerinDominique Israël-BietDavid M SmadjaPublished in: Stem cell reviews and reports (2020)
Idiopathic pulmonary fibrosis (IPF) is a severe, progressive and irreversible lung disease constantly associated with a major vascular remodeling process. Endothelial colony-forming cells (ECFCs) are human vasculogenic cells proposed as a cell therapy product or liquid biopsy in vascular disorders. Since the link between IPF and thrombosis has been largely proposed, the aim of our study was to explore hypercoagulability states in ECFCs from patients with IPF. We performed Thrombin generation assay (TGA) in cord blood (CB)-ECFCs, peripheral blood (PB)-ECFCs and IPF-ECFCs. Endogenous thrombin potential and peak were higher in IPF-ECFCs compared to CB-ECFCs and PB-ECFCs. As thrombin generation in ECFCs was increased, we evaluated anticoagulant proteins expressed on ECFCs membrane and identified thrombomodulin and EPCR. We found a significant decrease of both anticoagulant proteins at membrane using flow cytometry. This study is the first to examine ECFC thrombin generation in IPF. This new finding strongly argues for a role of ECFC in IPF pathophysiology and thrombotic related disorders in IPF. Graphical Abstract.
Keyphrases
- pulmonary embolism
- idiopathic pulmonary fibrosis
- interstitial lung disease
- cell therapy
- endothelial cells
- induced apoptosis
- cord blood
- flow cytometry
- peripheral blood
- end stage renal disease
- atrial fibrillation
- multiple sclerosis
- cell cycle arrest
- newly diagnosed
- chronic kidney disease
- stem cells
- oxidative stress
- prognostic factors
- cell proliferation
- early onset
- endoplasmic reticulum stress
- signaling pathway
- high throughput