Novel Antioxidant Peptides Derived from Feather Keratin Alleviate H 2 O 2 -Induced Oxidative Damage in HepG2 Cells via Keap1/Nrf2 Pathway.
Xiao-Dong PeiYi-Ning HeQing-Ling WuYan-Mei ZhangFan LiDao-Quan JiaoXiao-Ling LiuCheng-Hua WangPublished in: Journal of agricultural and food chemistry (2023)
Reactive oxygen species (ROS) are crucial for signal transduction and the maintenance of cellular homeostasis. However, superfluous ROS may engender chronic pathologies. Feather keratin is a promising new source of antioxidant peptides that can eliminate excess ROS and potentially treat oxidative stress-related diseases, but the underlying mechanisms have remained elusive. This study investigated the antioxidant effects and mechanisms against H 2 O 2 -induced oxidative damage in HepG2 cells of the two latest discovered antioxidant peptides, CRPCGPTP (CP-8) and ANSCNEPCVR (AR-10), first decrypted from feather keratin. The results revealed that CP-8 and AR-10 did not exhibit cytotoxicity to HepG2 cells while reducing intracellular ROS accumulation. Simultaneously, they enhanced the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), thus alleviating H 2 O 2 -induced cell apoptosis. Molecular docking analysis demonstrated that CP-8, AR-10 interacted well with the key amino acids in the Kelch domain of Keap1, thereby directly disrupting the Keap1-Nrf2 interaction. The peptides' biosafety and antioxidant activity via Keap1/Nrf2 signaling lay the groundwork for further animal studies and applications as functional food additives.
Keyphrases
- oxidative stress
- diabetic rats
- reactive oxygen species
- dna damage
- amino acid
- molecular docking
- cell death
- high glucose
- ischemia reperfusion injury
- drug induced
- induced apoptosis
- anti inflammatory
- protein protein
- nitric oxide
- hydrogen peroxide
- molecular dynamics simulations
- climate change
- risk assessment
- cell proliferation
- heat shock
- mass spectrometry