Cell Migration and Bone Formation from Mesenchymal Stem Cell Spheroids in Alginate Hydrogels Are Regulated by Adhesive Ligand Density.
Steve S HoAndrew T KeownBennett AddisonJ Kent LeachPublished in: Biomacromolecules (2017)
The adhesion and migration of cells entrapped in engineered materials is regulated by available adhesive ligands. Although mesenchymal stem cell (MSC) spheroids injected into damaged tissues promote repair, their transplantation in biomaterials which regulate cell migration from the aggregate may further enhance their therapeutic potential. Alginate hydrogels were modified with Arginine-Glycine-Aspartic acid (RGD) at increasing concentrations, and osteogenically induced human MSC spheroids were entrapped to assess cell migration, survival, and differentiation. Cell migration was greater from MSC spheroids in alginate modified with low RGD levels, while the osteogenic potential was higher for spheroids entrapped in unmodified or high RGD density gels in vitro. Upon ectopic implantation, microCT and immunohistochemistry revealed extensive osteogenesis in unmodified and high RGD density gels compared to low RGD density gels. These data suggest that restriction of MSC migration from spheroids correlates with enhanced spheroid osteogenic potential, representing a novel tool for bone tissue engineering.
Keyphrases
- cell migration
- tissue engineering
- mesenchymal stem cells
- bone marrow
- wound healing
- umbilical cord
- induced apoptosis
- endothelial cells
- cell therapy
- gene expression
- machine learning
- risk assessment
- high glucose
- oxidative stress
- big data
- hyaluronic acid
- human health
- stem cells
- body composition
- cystic fibrosis
- staphylococcus aureus
- amino acid
- deep learning
- signaling pathway
- electronic health record
- pseudomonas aeruginosa
- artificial intelligence
- candida albicans
- cell adhesion
- bone loss