A synergistic anti-bacterial and anti-adhesion activity of tea tree ( Melaleuca alternifolia ) and lemon eucalyptus tree ( Eucalyptus citriodora Hook) essential oils on Legionella pneumophila .

Legionella pneumophila is a Gram-negative bacterial pathogen that colonizes natural and artificial water systems and has the ability to form a biofilm. The biofilm protects L. pneumophila from various environmental factors and makes it more resistant to chlorine-based disinfectants. This study investigated the anti-bacterial properties of tea tree ( Melaleuca alternifolia (Maiden and Betche) Cheel) oil and lemon eucalyptus tree ( Eucalyptus citriodora Hook) essential oils (EOs) and their synergistic, additive inhibitory and anti-adhesive effects against L. pneumophila biofilm formation on polystyrene. The minimum effective concentration (MEC) for tea tree is 12.8 mg ml -1 and for lemon eucalyptus tree EO 6.4 mg ml -1 . In the checkerboard assay, different combinations of these two EO show synergistic and additive anti-microbial activity. The minimum anti-adhesive concentration (MAC) for tea tree is 12.8 mg ml -1 and for lemon eucalyptus tree EO 6.4 mg ml -1 . A combination of 3.2 mg ml -1 tea tree EO and 0.8 mg ml -1 lemon eucalyptus tree EO showed the strongest anti-adhesive effect against L. pneumophila on polystyrene. The tested oils and their combination showed intriguing potential to inhibit L. pneumophila biofilm formation.