Immunosuppressive Polymeric Nanoparticles Targeting Dendritic Cells Alleviate Lupus Disease in Fcgr2b -/- Mice by Mediating Antigen-Specific Immune Tolerance.
Phuriwat KhiewkamropChamraj KaewraemruaenChonnavee ManipunteeChalathan SaengruengritNumpon InsinAsada LeelahavanichkulWarerat KaewduangduenOpor SonpoungKasirapat Ariya-AnandechNattiya HirankarnPatcharee RitprajakPublished in: International journal of molecular sciences (2023)
Dendritic cells (DCs) are the most potent antigen-presenting cells that have multifaceted functions in the control of immune activation and tolerance. Hyperresponsiveness and altered tolerogenicity of DCs contribute to the development and pathogenesis of system lupus erythematosus (SLE); therefore, DC-targeted therapies aimed at inducing specific immune tolerance have become of great importance for the treatment of SLE. This study developed a new nanoparticle (NP) containing a biodegradable PDMAEMA-PLGA copolymer for target-oriented delivery to DCs in situ. PDMAEMA-PLGA NPs provided sustained drug release and exhibited immunosuppressive activity in FLT3L and GM-CSF-derived bone marrow in conventional DCs (BM-cDCs). PDMAEMA-PLGA NPs improved dexamethasone capability to convert wild-type and Fcgr2b -/- BM-cDCs from an immunogenic to tolerogenic state, and BM-cDCs treated with dexamethasone-incorporated PDMAEMA-PLGA NPs (Dex-NPs) efficiently mediated regulatory T cell (Treg) expansion in vitro. Dex-NP therapy potentially alleviated lupus disease in Fcgr2b -/- mice by mediating Foxp3 + Treg expansion in an antigen-specific manner. Our findings substantiate the superior efficacy of DC-targeted therapy using the PDMAEMA-PLGA NP delivery system and provide further support for clinical development as a potential therapy for SLE. Furthermore, PDMAEMA-PLGA NP may be a versatile platform for DC-targeted therapy to induce antigen-specific immune tolerance to unwanted immune responses that occur in autoimmune disease, allergy, and transplant rejection.
Keyphrases
- drug release
- dendritic cells
- systemic lupus erythematosus
- drug delivery
- disease activity
- immune response
- regulatory t cells
- wild type
- cancer therapy
- bone marrow
- rheumatoid arthritis
- low dose
- induced apoptosis
- transcription factor
- acute myeloid leukemia
- high dose
- bone regeneration
- high fat diet induced
- adipose tissue
- type diabetes
- metabolic syndrome
- oxidative stress
- stem cells
- oxide nanoparticles
- cell death
- tyrosine kinase
- signaling pathway
- inflammatory response
- atopic dermatitis
- toll like receptor
- case report