Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM).
Myriam HollMarie-Lena RaschLucas BeckerAnna-Lena KellerLaura Schultze-RhonhofFelix RuoffMarkus TemplinSilke KellerFelix NeisFranziska KeßlerJürgen AndressCornelia BachmannBernhard KrämerKatja Schenke-LaylandSara Yvonne BruckerJulia MarziMartin WeissPublished in: Biomedicines (2022)
Postoperative abdominal adhesions are responsible for serious clinical disorders. Administration of plasma-activated media (PAM) to cell type-specific modulated proliferation and protein biosynthesis is a promising therapeutic strategy to prevent pathological cell responses in the context of wound healing disorders. We analyzed PAM as a therapeutic option based on cell type-specific anti-adhesive responses. Primary human peritoneal fibroblasts and mesothelial cells were isolated, characterized and exposed to different PAM dosages. Cell type-specific PAM effects on different cell components were identified by contact- and marker-independent Raman imaging, followed by thorough validation by specific molecular biological methods. The investigation revealed cell type-specific molecular responses after PAM treatment, including significant cell growth retardation in peritoneal fibroblasts due to transient DNA damage, cell cycle arrest and apoptosis. We identified a therapeutic dose window wherein specifically pro-adhesive peritoneal fibroblasts were targeted, whereas peritoneal mesothelial cells retained their anti-adhesive potential of epithelial wound closure. Finally, we demonstrate that PAM treatment of peritoneal fibroblasts reduced the expression and secretion of pro-adhesive cytokines and extracellular matrix proteins. Altogether, we provide insights into biochemical PAM mechanisms which lead to cell type-specific pro-therapeutic cell responses. This may open the door for the prevention of pro-adhesive clinical disorders.
Keyphrases
- cell cycle arrest
- extracellular matrix
- single cell
- cell death
- dna damage
- cell therapy
- pi k akt
- oxidative stress
- anti inflammatory
- endothelial cells
- high resolution
- wound healing
- minimally invasive
- pseudomonas aeruginosa
- small molecule
- cell proliferation
- mesenchymal stem cells
- signaling pathway
- brain injury
- cerebral ischemia
- cell migration
- amino acid