Inhibition of Phytosterol Biosynthesis by Azasterols.

Sylvain Darnet Laetitia B B MartinPierre MercierFranz BracherPhilippe GeoffroyHubert Schaller

Published in: Molecules (Basel, Switzerland) (2020)

Inhibitors of enzymes in essential cellular pathways are potent probes to decipher intricate physiological functions of biomolecules. The analysis of Arabidopsis thaliana sterol profiles upon treatment with a series of azasterols reveals a specific in vivo inhibition of SMT2, a plant sterol-C-methyltransferase acting as a branch point between the campesterol and sitosterol biosynthetic segments in the pathway. Side chain azasteroids that modify sitosterol homeostasis help to refine its particular function in plant development.

Keyphrases

arabidopsis thaliana
cell wall
small molecule
single molecule
fluorescence imaging
living cells
smoking cessation
fluorescent probe