High efficiency method of detection and isolation of neuraminidase inhibitor resistant influenza viruses by fluorescence sialidase imaging.
Yuuki KurebayashiPublished in: The Japanese journal of antibiotics (2018)
Influenza A and B viruses possess an enzyme "sialidase" that cleavages terminal sialic acid from glycochains. These viral sialidase proteins are highly expressed on the virus infected cells. We developed sialidase imaging probe "BTP3-Neu5Ac" that enables histochemical fluorescence staining of sialidase activity. BTP3-Neu5Ac was able to perform speedy and easy fluorescence imaging of these virus infected cells, with no needs of specific antibody and cell fixation. In addition, combination use of anti-influenza drugs (sialidase inhibitors) and BTP3-Neu5Ac resulted in selective fluorescence imaging for detection and high-efficiency isolation of drug-resistant virus. Fluorescence imaging of drug-resistant virus will be a powerful method for study of the drug-resistance mechanism, for monitoring of drug-resistant viruses. A novel tool for fluorescence imaging of viral sialidase activity is described in this review.
Keyphrases
- fluorescence imaging
- drug resistant
- high efficiency
- multidrug resistant
- photodynamic therapy
- acinetobacter baumannii
- induced apoptosis
- cell cycle arrest
- sars cov
- single molecule
- single cell
- minimally invasive
- cell therapy
- quantum dots
- endoplasmic reticulum stress
- cystic fibrosis
- signaling pathway
- pseudomonas aeruginosa
- cell surface