Comparisons between the ON- and OFF-edge motion pathways in the Drosophila brain.
Kazunori ShinomiyaGary HuangZhiyuan LuToufiq ParagC Shan XuRoxanne AnicetoNamra AnsariNatasha CheathamShirley LauchieErika NeaceOmotara OgundeyiChristopher OrdishDavid PeelAya ShinomiyaClaire SmithSatoko TakemuraIris TalebiPatricia K RivlinAljoscha NernLouis K SchefferStephen M PlazaIan A MeinertzhagenPublished in: eLife (2019)
Understanding the circuit mechanisms behind motion detection is a long-standing question in visual neuroscience. In Drosophila melanogaster, recently discovered synapse-level connectomes in the optic lobe, particularly in ON-pathway (T4) receptive-field circuits, in concert with physiological studies, suggest a motion model that is increasingly intricate when compared with the ubiquitous Hassenstein-Reichardt model. By contrast, our knowledge of OFF-pathway (T5) has been incomplete. Here, we present a conclusive and comprehensive connectome that, for the first time, integrates detailed connectivity information for inputs to both the T4 and T5 pathways in a single EM dataset covering the entire optic lobe. With novel reconstruction methods using automated synapse prediction suited to such a large connectome, we successfully corroborate previous findings in the T4 pathway and comprehensively identify inputs and receptive fields for T5. Although the two pathways are probably evolutionarily linked and exhibit many similarities, we uncover interesting differences and interactions that may underlie their distinct functional properties.
Keyphrases
- resting state
- functional connectivity
- drosophila melanogaster
- optical coherence tomography
- white matter
- high speed
- magnetic resonance
- healthcare
- machine learning
- optic nerve
- deep learning
- multiple sclerosis
- health information
- subarachnoid hemorrhage
- mass spectrometry
- brain injury
- cerebral ischemia
- case control
- label free