Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction.
Marlene Louise QuaadePratibha DhumaleSimon Gabriel Comerma SteffensenHans Christian BeckEva Bang HarvaldCharlotte Harken JensenLars LundDitte Caroline AndersenSøren Paludan SheikhPublished in: International journal of molecular sciences (2022)
Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as well as in recent Pase 1 trials in humans. However, some studies indicate that diabetes negatively affects the mesenchymal stem cell pool, implying that ASCs from T2DM patients could have impaired regenerative capacity. Here, we directly compared ASCs from age-matched diabetic Goto-Kakizaki (ASC GK ) and non-diabetic wild type rats (ASC WT ) with regard to their phenotypes, proteomes and ability to rescue ED in normal rats. Despite ASC GK exhibiting a slightly lower proliferation rate, ASC GK and ASC WT proteomes were more or less identical, and after injections to corpus cavernosum they were equally efficient in restoring erectile function in a rat ED model entailing bilateral nerve crush injury. Moreover, molecular analysis of the corpus cavernosum tissue revealed that both ASC GK and ASC WT treated rats had increased induction of genes involved in recovering endothelial function. Thus, our finding argues that T2DM does not appear to be a limiting factor for autologous adipose stem cell therapy when correcting for ED.
Keyphrases
- cell therapy
- nlrp inflammasome
- emergency department
- mesenchymal stem cells
- stem cells
- type diabetes
- diabetic rats
- wound healing
- oxidative stress
- wild type
- glycemic control
- newly diagnosed
- cardiovascular disease
- ejection fraction
- adipose tissue
- bone marrow
- signaling pathway
- squamous cell carcinoma
- case report
- insulin resistance
- metabolic syndrome
- young adults
- platelet rich plasma
- chronic kidney disease
- ultrasound guided
- patient reported outcomes
- replacement therapy
- optical coherence tomography