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Encoding, Decoding, and Rendering Information in DNA Nanoswitch Libraries.

Hannah TalbotKen HalvorsenArun Richard Chandrasekaran
Published in: ACS synthetic biology (2022)
DNA-based construction allows the creation of molecular devices that are useful in information storage and processing. Here, we combine the programmability of DNA nanoswitches and stimuli-responsive conformational changes to demonstrate information encoding and graphical readout using gel electrophoresis. We encoded information as 5-bit binary codes for alphanumeric characters using a combination of DNA and RNA inputs that can be decoded using molecular stimuli such as a ribonuclease. We also show that a similar strategy can be used for graphical visual readout of alphabets on an agarose gel, information that is encoded by nucleic acids and decoded by a ribonuclease. Our method of information encoding and processing could be combined with DNA actuation for molecular computation and diagnostics that require a nonarbitrary visual readout.
Keyphrases
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  • nucleic acid
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  • molecular dynamics
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  • hyaluronic acid