How long does the biological effect of a red light-emitting diode last on adipose-derived mesenchymal stem cells?
Vitor Pocani da RochaBarbara Sampaio Dias Martins MansanoCarolina Fernanda Chaves Dos SantosIghor Luiz Azevedo TeixeiraHelenita Antonia de OliveiraStella Sousa VieiraEdnei Luiz AntonioMaria Cristina de Oliveira IzarFrancisco Antonio Helfenstein FonsecaAndrey Jorge SerraPublished in: Photochemistry and photobiology (2024)
This research investigated the duration of the influence of red light-emitting diodes (LED, 630 nm; output power: 2452.5 mW; laser beam: 163.5 cm 2 ; irradiance: 15 mW/cm 2 ; radiant exposure: 4 J/cm 2 ) on different periods after irradiation (6, 12, 24, 48, and 72 h) on adipose-derived mesenchymal stem cells' (AdMSCs) metabolism and paracrine factors. AdMSCs were irradiated three times every 48 h. Twenty-four hours after the last irradiation, there was a higher MTT absorbance, followed by a decrease after 48 h. The cells' secretome showed increased levels of IL-6 and VEGF after 12 and 24 h, but this was reversed after 48 h. Additionally, LED irradiation resulted in higher levels of nitrite and did not affect oxidative stress markers. LED irradiation had significant effects on AdMSCs after 24 h compared to other groups and its control group.
Keyphrases
- light emitting
- mesenchymal stem cells
- oxidative stress
- induced apoptosis
- radiation induced
- adipose tissue
- vascular endothelial growth factor
- photodynamic therapy
- endothelial cells
- umbilical cord
- insulin resistance
- type diabetes
- cell death
- signaling pathway
- radiation therapy
- cell proliferation
- ischemia reperfusion injury
- high resolution
- skeletal muscle
- metabolic syndrome
- mass spectrometry
- single molecule
- cell therapy
- pi k akt