In recent years, the hepatotoxicity of Polygoni Multiflora Radix (PMR) has attracted increased research interest. Some studies suggest that anthraquinone may be the main hepatotoxic component. Most of the relevant studies have focused on the mononuclear anthraquinone component rather than binuclear anthraquinones. The hepatotoxicity of dinuclear anthraquinone (dianthrone) was investigated in a cell-based model. Next, a method for the determination of six free and total dianthonones in PMR and PMR Praeparata (PMRP) was established using ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS), which was then used to analyze the collected samples. The data show that four binuclear anthraquinone compounds were hepatotoxic and may be potential toxicity indicators for the safety evaluation of PMR and PMRP. Herein, we provide a theoretical basis for the improvement of PMRP quality standards.
Keyphrases
- tandem mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- mass spectrometry
- simultaneous determination
- high performance liquid chromatography
- solid phase extraction
- quality control
- high resolution mass spectrometry
- gas chromatography
- ms ms
- drug induced
- liquid chromatography tandem mass spectrometry
- high resolution
- case control
- single cell
- molecularly imprinted
- human health
- oxidative stress
- peripheral blood
- electronic health record
- big data
- bone marrow
- risk assessment
- capillary electrophoresis
- machine learning
- stem cells
- data analysis
- climate change