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A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.

Stian OlsenKirsten Krause
Published in: Plant methods (2019)
Plants can be prepared quickly and easily for laser microdissection by direct sectioning of fresh tissue followed by dehydration on glass slides. We show that RNA isolated from material treated in this manner maintains high quality and enables the investigation of differential gene expression at a high morphological resolution.
Keyphrases
  • gene expression
  • genome wide identification
  • dna methylation
  • high speed
  • genome wide
  • copy number
  • minimally invasive
  • loop mediated isothermal amplification
  • mass spectrometry
  • newly diagnosed
  • nucleic acid