Inflammation protein quantification by multiple reaction monitoring mass spectrometry in lipopolysaccharide-stimulated THP-1 cells.
Anna IllianoGabriella PintoRosa GaglioneAngela ArcielloAngela AmoresanoPublished in: Rapid communications in mass spectrometry : RCM (2021)
The developed MRM-MS method allowed the quantification of TNF-α, INF-γ, IL-8 and IL-10 along a time-course from 2 to 24 h. Hence, a trace of the kinetics of the inflammatory response in THP-1 cells upon stimulation with E. coli LPSs was obtained. Finally, the extensibility of the developed MRM method to serum samples and other matrices demonstrated the versatility of the approach and the possibility to quantify multiple target proteins in different biological samples by using a few microliters in a single analysis.
Keyphrases
- inflammatory response
- mass spectrometry
- induced apoptosis
- cell cycle arrest
- oxidative stress
- escherichia coli
- rheumatoid arthritis
- multiple sclerosis
- liquid chromatography
- endoplasmic reticulum stress
- lipopolysaccharide induced
- lps induced
- signaling pathway
- high resolution
- risk assessment
- heavy metals
- immune response
- cell proliferation
- protein protein
- small molecule
- simultaneous determination