Login / Signup

The Molecular Architecture of the Nuclear Basket.

Digvijay SinghNeelesh SoniJoshua HutchingsIgnacia EcheverriaFarhaz ShaikhMadeleine DuquetteSergey SuslovZhixun LiTrevor Van EeuwenKelly MolloyYi ShiJunjie WangQiang GuoBrian T ChaitJavier Fernandez-MartinezMichael P RoutAndrej SaliElizabeth Villa
Published in: bioRxiv : the preprint server for biology (2024)
The nuclear pore complex (NPC) is the sole mediator of nucleocytoplasmic transport. Despite great advances in understanding its conserved core architecture, the peripheral regions can exhibit considerable variation within and between species. One such structure is the cage-like nuclear basket. Despite its crucial roles in mRNA surveillance and chromatin organization, an architectural understanding has remained elusive. Using in-cell cryo-electron tomography and subtomogram analysis, we explored the NPC's structural variations and the nuclear basket across fungi (yeast; S. cerevisiae ), mammals (mouse; M. musculus ), and protozoa ( T. gondii ). Using integrative structural modeling, we computed a model of the basket in yeast and mammals that revealed how a hub of Nups in the nuclear ring binds to basket-forming Mlp/Tpr proteins: the coiled-coil domains of Mlp/Tpr form the struts of the basket, while their unstructured termini constitute the basket distal densities, which potentially serve as a docking site for mRNA preprocessing before nucleocytoplasmic transport.
Keyphrases
  • single cell
  • transcription factor
  • gene expression
  • dna damage
  • stem cells
  • magnetic resonance
  • bone marrow
  • mesenchymal stem cells
  • mass spectrometry
  • dna methylation
  • small molecule
  • electron microscopy