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Dawn and dusk peaks of outer segment phagocytosis, and visual cycle function require Rab28.

Ailis L MoranStephen P CarterJoanna J KaylorZhichun JiangSanne BroekmanEugene T DillonAlicia Gómez SánchezSajal K MinhasErwin van WijkRoxana A RaduGabriel H TravisMichelle CareyOliver E BlacqueBreandán N Kennedy
Published in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022)
RAB28 is a farnesylated, ciliary G-protein. Patient variants in RAB28 are causative of autosomal recessive cone-rod dystrophy (CRD), an inherited human blindness. In rodent and zebrafish models, the absence of Rab28 results in diminished dawn, photoreceptor, outer segment phagocytosis (OSP). Here, we demonstrate that Rab28 is also required for dusk peaks of OSP, but not for basal OSP levels. This study further elucidated the molecular mechanisms by which Rab28 controls OSP and inherited blindness. Proteomic profiling identified factors whose expression in the eye or whose expression at dawn and dusk peaks of OSP is dysregulated by loss of Rab28. Notably, transgenic overexpression of Rab28, solely in zebrafish cones, rescues the OSP defect in rab28 KO fish, suggesting rab28 gene replacement in cone photoreceptors is sufficient to regulate Rab28-OSP. Rab28 loss also perturbs function of the visual cycle as retinoid levels of 11-cRAL, 11cRP, and atRP are significantly reduced in larval and adult rab28 KO retinae (p < .05). These data give further understanding on the molecular mechanisms of RAB28-associated CRD, highlighting roles of Rab28 in both peaks of OSP, in vitamin A metabolism and in retinoid recycling.
Keyphrases
  • gene expression
  • cell proliferation
  • machine learning
  • dna methylation
  • copy number
  • early onset
  • autism spectrum disorder
  • single cell
  • data analysis