Evaluation of fresh, frozen, and lyophilized fecal samples by SPME and derivatization methods using GC×GC-TOFMS.
Seo Lin NamKieran Tarazona CarrilloA Paulina de la MataOlle M de BruinEvgueni DoukhanineJames J HarynukPublished in: Metabolomics : Official journal of the Metabolomic Society (2022)
A comprehensive comparison of the sample handling conditions provides a deeper understanding of the physicochemical changes that occur within the samples during freezing and lyophilization. Based on our results, snap-freezing at -80 °C would be preferred over lyophilization for handling samples in the field of fecal metabolomics as this imparts the least change from the fresh condition.