TopFD: A Proteoform Feature Detection Tool for Top-Down Proteomics.
Abdul Rehman BasharatYong ZangLiangliang SunXiaowen LiuPublished in: Analytical chemistry (2023)
Top-down liquid chromatography-mass spectrometry (LC-MS) analyzes intact proteoforms and generates mass spectra containing peaks of proteoforms with various isotopic compositions, charge states, and retention times. An essential step in top-down MS data analysis is proteoform feature detection, which aims to group these peaks into peak sets (features), each containing all peaks of a proteoform. Accurate protein feature detection enhances the accuracy in MS-based proteoform identification and quantification. Here, we present TopFD, a software tool for top-down MS feature detection that integrates algorithms for proteoform feature detection, feature boundary refinement, and machine learning models for proteoform feature evaluation. We performed extensive benchmarking of TopFD, ProMex, FlashDeconv, and Xtract using seven top-down MS data sets and demonstrated that TopFD outperforms other tools in feature accuracy, reproducibility, and feature abundance reproducibility.
Keyphrases
- machine learning
- mass spectrometry
- deep learning
- liquid chromatography
- loop mediated isothermal amplification
- data analysis
- artificial intelligence
- multiple sclerosis
- label free
- big data
- ms ms
- real time pcr
- high performance liquid chromatography
- gas chromatography
- neural network
- capillary electrophoresis
- quantum dots
- binding protein
- wastewater treatment
- tandem mass spectrometry
- sensitive detection