Intrathecal Injection of Autologous Mesenchymal Stem-Cell-Derived Extracellular Vesicles in Spinal Cord Injury: A Feasibility Study in Pigs.
Ilya ShulmanTatyana AgeevaAlexander KostennikovSergei OgurcovLeysan TazetdinovaIlyas KabdeshAlexander RogozhinIlnur GanievAlbert Anatolyevich RizvanovYana MukhamedshinaPublished in: International journal of molecular sciences (2023)
Spinal cord injury (SCI) remains one of the current medical and social problems, as it causes deep disability in patients. The use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) is one strategy for stimulating the post-traumatic recovery of the structure and function of the spinal cord. Here, we chose an optimal method for obtaining cytochalasin B-induced EVs, including steps with active vortex mixing for 60 s and subsequent filtration to remove nuclei and disorganized inclusions. The therapeutic potential of repeated intrathecal injection of autologous MSC-derived EVs in the subacute period of pig contused SCI was also evaluated for the first time. In this study, we observed the partial restoration of locomotor activity by stimulating the remyelination of axons and timely reperfusion of nervous tissue.
Keyphrases
- spinal cord injury
- spinal cord
- bone marrow
- mesenchymal stem cells
- neuropathic pain
- cell therapy
- end stage renal disease
- mental health
- healthcare
- newly diagnosed
- ejection fraction
- chronic kidney disease
- high glucose
- peritoneal dialysis
- acute myocardial infarction
- ultrasound guided
- platelet rich plasma
- heart failure
- diabetic rats
- endothelial cells
- drug induced
- acute coronary syndrome
- patient reported