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Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition.

Magdalena E PotokYafei WangLinhao XuZhenhui ZhongWanlu LiuSuhua FengBilguudei NaranbaatarShima RayatpishehZonghua WangJames A WohlschlegelIsrael AusínSteven E Jacobsen
Published in: Nature communications (2019)
Deposition of the histone variant H2A.Z by the SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is important for gene regulation in eukaryotes, but the composition of the Arabidopsis SWR1-C has not been thoroughly characterized. Here, we aim to identify interacting partners of a conserved Arabidopsis SWR1 subunit ACTIN-RELATED PROTEIN 6 (ARP6). We isolate nine predicted components and identify additional interactors implicated in histone acetylation and chromatin biology. One of the interacting partners, methyl-CpG-binding domain 9 (MBD9), also strongly interacts with the Imitation SWItch (ISWI) chromatin remodeling complex. MBD9 is required for deposition of H2A.Z at a distinct subset of ARP6-dependent loci. MBD9 is preferentially bound to nucleosome-depleted regions at the 5' ends of genes containing high levels of activating histone marks. These data suggest that MBD9 is a SWR1-C interacting protein required for H2A.Z deposition at a subset of actively transcribing genes.
Keyphrases
  • genome wide
  • dna methylation
  • transcription factor
  • dna binding
  • genome wide identification
  • gene expression
  • protein kinase
  • binding protein
  • dna damage
  • plant growth
  • oxidative stress
  • big data
  • data analysis