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The use of cell and larval assays to identify target genes for RNA interference-meditated control of the Australian sheep blowfly (Lucilia cuprina).

Yunjia YangBing ZhangJiaxi YongPeter JamesZhi Ping XuNeena MitterTimothy J MahonyKarishma T Mody
Published in: Pest management science (2024)
This study confirms that the novel BFEC cell line can be used to improve the efficacy of dsRNA-mediated screening to accelerate the identification of potential target genes in the development of RNAi mediated control approaches for L. cuprina. The research models established in this study are encouraging with respect to the use of RNAi as a blowfly control method, however further improvement and validation are required for field applicationsnot prefect, and could be ongoing developing. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Keyphrases
  • genome wide
  • stem cells
  • single cell
  • bioinformatics analysis
  • bone marrow
  • climate change
  • zika virus
  • genome wide identification
  • human health
  • nucleic acid