Dual analysis of wild-type and attenuated Orf virus and host cell transcriptomes revealed novel virus-host cell interactions.
Xiao-Ting YaoTian JingQingru GengMing PangXuanduo ZhaoShaofei LiDekun ChenWen-Tao MaPublished in: mSphere (2023)
(ORFV) are live attenuated vaccines, which present a potential risk of reversion to virulence. Therefore, understanding the pathogenic mechanisms of different virulent strains of ORFV and host immune responses triggered by these viruses is crucial for developing new vaccines and interventions. In this study, we found that the attenuated strain downregulates the host innate immune response and antiviral activity. In addition, we noted that the wild-type strain can induce the immune response pattern centered on interferon-stimulated genes and interferon regulatory factor gene family. We predicted that STAT1 and STAT2 are the main transcription factors upstream of target gene promoters through gene regulatory networks and exert significant regulatory effects on co-expressed genes. Our study elucidated the complex interaction between ORFV strains and host cell immune responses, providing new insights into vaccine research for ORFV.
Keyphrases
- immune response
- wild type
- single cell
- dendritic cells
- transcription factor
- escherichia coli
- cell therapy
- toll like receptor
- genome wide
- genome wide identification
- cell proliferation
- physical activity
- staphylococcus aureus
- pseudomonas aeruginosa
- dna methylation
- mesenchymal stem cells
- climate change
- antimicrobial resistance
- cystic fibrosis
- genome wide analysis