Synchronized HIV assembly by tunable PIP2 changes reveals PIP2 requirement for stable Gag anchoring.
Frauke MückschVibor LaketaBarbara MüllerCarsten SchultzHans-Georg KräusslichPublished in: eLife (2017)
HIV-1 assembles at the plasma membrane (PM) of infected cells. PM association of the main structural protein Gag depends on its myristoylated MA domain and PM PI(4,5)P2. Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 completely prevents Gag PM targeting and assembly site formation. Unexpectedly, PI(4,5)P2 depletion also caused loss of pre-assembled Gag lattices from the PM. Subsequent restoration of PM PI(4,5)P2 reinduced assembly site formation even in the absence of new protein synthesis, indicating that the dissociated Gag molecules remained assembly competent. These results reveal an important role of PI(4,5)P2 for HIV-1 morphogenesis beyond Gag recruitment to the PM and suggest a dynamic equilibrium of Gag-lipid interactions. Furthermore, they establish an experimental system that permits synchronized induction of HIV-1 assembly leading to induced production of infectious virions by targeted modulation of Gag PM targeting.
Keyphrases
- particulate matter
- air pollution
- polycyclic aromatic hydrocarbons
- antiretroviral therapy
- hiv positive
- heavy metals
- hiv infected
- hiv testing
- human immunodeficiency virus
- hepatitis c virus
- water soluble
- hiv aids
- living cells
- men who have sex with men
- cancer therapy
- induced apoptosis
- gene expression
- fluorescent probe
- molecular dynamics simulations
- cell death
- south africa
- molecular dynamics
- risk assessment
- mouse model
- genome wide
- single molecule
- endoplasmic reticulum stress
- endothelial cells
- protein protein
- binding protein
- cell cycle arrest
- signaling pathway