Herein, an ultrasensitive electrochemical biosensor is proposed for the quantification of the Flu A virus biomarker DNA (fDNA), and is based on loop-mediated isothermal amplification-generated hydrogen ions (LAMP-H+) which induce the formation of the dimer i-motif structure (DiMS) for signal transduction, coupled with exonuclease III (ExoIII)-assisted DNA walking for signal dual-amplification.
Keyphrases
- loop mediated isothermal amplification
- label free
- sensitive detection
- quantum dots
- gold nanoparticles
- circulating tumor
- cell free
- nucleic acid
- single molecule
- molecularly imprinted
- ionic liquid
- high glucose
- diabetic rats
- drug induced
- endothelial cells
- mass spectrometry
- aqueous solution
- water soluble
- simultaneous determination