An amino acid-based supramolecular nanozyme by coordination self-assembly for cascade catalysis and enhanced chemodynamic therapy towards biomedical applications.

The clinical translation of chemodynamic therapy has been highly obstructed by the insufficient intracellular H 2 O 2 level in diseased tissues. Herein, we developed a supramolecular nanozyme through a facile one-step cooperative coordination self-assembly of an amphipathic amino acid and glucose oxidase (GOx) in the presence of Fe 2+ . The results demonstrated that the supramolecular nanozyme possessed cascade enzymatic activity ( i.e. , GOx and peroxidase), which could amplify the killing efficacy of hydroxyl radicals (˙OH) via self-supplying H 2 O 2 , finally achieving synergistic starvation-chemodynamic cancer therapy in vitro . Additionally, this cascade nanozyme also exhibited highly effective antibacterial activity on Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) without the need for additional H 2 O 2 . This work provided a promising strategy for the design and development of nanozymes for future biomedical applications.