Rotaxane probes for protease detection by 129Xe hyperCEST NMR.

Clancy C Slack Joel A FinbloomKeunhong JeongCarson J BrunsDavid E WemmerAlexander PinesMatthew B Francis

Published in: Chemical communications (Cambridge, England) (2018)

We report a CB6 rotaxane for the 129Xe hyperCEST NMR detection of matrix metalloprotease 2 (MMP-2) activity. MMP-2 is overexpressed in cancer tissue, and hence is a cancer marker. A peptide containing an MMP-2 recognition sequence was incorporated into the rotaxane, synthesized via CB6-promoted click chemistry. Upon cleavage of the rotaxane by MMP-2, CB6 became accessible for 129Xe@CB6 interactions, leading to protease-responsive hyperCEST activation.

Keyphrases

papillary thyroid
cell migration
magnetic resonance
squamous cell
high resolution
loop mediated isothermal amplification
solid state
label free
small molecule
lymph node metastasis
single molecule
drug delivery
fluorescence imaging
dna binding
amino acid
sensitive detection