RESPAN: an accurate, unbiased and automated pipeline for analysis of dendritic morphology and dendritic spine mapping.

RESPAN is a new automated pipeline for restoration and segmentation of several morphological features of neurons including soma, dendritic shaft, and dendritic spines from various in light microscopy imaging datasets.The RESPAN processing pipeline includes data preparation, image restoration, image segmentation, and subsequent visualization and plotting of the resulting data, providing a comprehensive, automated solution for mapping dendrites and dendritic spines.By providing clear guidance and an easy to use GUI for using RESPAN and additional Python-based tools, including nnU-Net and SelfNet, we ensure state-of-the-art automated neuron analysis is broadly accessible for a range of diverse datasets and applications.