Cutting Edge: Innate Immune Augmenting Vesicular Stomatitis Virus Expressing Zika Virus Proteins Confers Protective Immunity.
Dillon M BetancourtNina M G P de QueirozTianli XiaJeonghyun AhnGlen N BarberPublished in: Journal of immunology (Baltimore, Md. : 1950) (2017)
Zika virus (ZIKV) has become a serious public health concern because of its link to brain damage in developing human fetuses. Recombinant vesicular stomatitis virus (rVSV) was shown to be a highly effective and safe vector for the delivery of foreign immunogens for vaccine purposes. In this study, we generated rVSVs (wild-type and attenuated VSV with mutated matrix protein [VSVm] versions) that express either the full length ZIKV envelope protein (ZENV) alone or include the ZENV precursor to the membrane protein upstream of the envelope protein, and our rVSV-ZIKV constructs showed efficient immunogenicity in murine models. We also demonstrated maternal protective immunity in challenged newborn mice born to female mice vaccinated with VSVm-ZENV containing the transmembrane domain. Our data indicate that rVSVm may be a suitable strategy for the design of effective vaccines against ZIKV.
Keyphrases
- zika virus
- wild type
- dengue virus
- public health
- aedes aegypti
- innate immune
- protein protein
- endothelial cells
- amino acid
- high fat diet induced
- gestational age
- oxidative stress
- multiple sclerosis
- machine learning
- type diabetes
- small molecule
- adipose tissue
- body mass index
- artificial intelligence
- induced pluripotent stem cells
- subarachnoid hemorrhage
- blood brain barrier
- skeletal muscle