Polydopamine decorated MoS 2 nanosheet based electrochemical immunosensor for sensitive detection of SARS-CoV-2 nucleocapsid protein in clinical samples.
Shalu YadavMohd Abubakar SadiquePushpesh RanjanMohd Akram KhanSathish NatarajanAvanish K SrivastavaPublished in: Journal of materials chemistry. B (2022)
The outbreak of the highly contagious disease COVID-19, which is triggered by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), demands a rapid, low-cost, and highly sensitive immunosensor that can detect and identify the virus efficiently. Here, an electrochemical immunosensor based on a nanocomposite consisting of molybdenum disulfide nanosheets decorated with polydopamine (MoS 2 -PDA) is developed for highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein). The MoS 2 -PDA nanocomposite possesses various hydroxyl and amine groups that have excellent chemistry with crosslinkers and act as adhesive agents to bind with the working electrode surface. Furthermore, the optical, functional, structural, vibrational, and morphological properties of the MoS 2 -PDA nanocomposite are studied using various characterization techniques such as UV-vis, FTIR, and Raman spectroscopies, XRD, and TEM. The electrochemical immunosensor is fabricated by functionalizing the MoS 2 -PDA nanocomposite with anti-SARS-CoV-2 nucleocapsid IgG antibody (Ab) and has a very high sensitivity against the N protein with a linear range between 10 ag mL -1 and 100 ng mL -1 . The electrochemical immunosensor exhibits a lowest limit of detection (LOD) of 2.80 ag mL -1 and a limit of quantification (LOQ) of 8.48 ag mL -1 via electrochemical impedance spectroscopy (EIS). Furthermore, the electrochemical immunosensor is successfully employed to detect the N protein in nasopharyngeal swab specimens and displays good consistency with the conventional RT-PCR test results. The results show that the MoS 2 -PDA nanocomposite-based electrochemical platform can serve as a highly sensitive and selective detector of N protein and will pave the way for the development of a point-of-care (POC) electrochemical immunosensor for rapid detection of other infectious viruses.
Keyphrases
- quantum dots
- sensitive detection
- sars cov
- respiratory syndrome coronavirus
- label free
- reduced graphene oxide
- gold nanoparticles
- loop mediated isothermal amplification
- molecularly imprinted
- ionic liquid
- coronavirus disease
- energy transfer
- visible light
- highly efficient
- protein protein
- binding protein
- computed tomography
- magnetic resonance imaging
- single cell
- magnetic resonance
- mass spectrometry
- single molecule
- ultrasound guided
- high throughput
- image quality
- dual energy