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SARS-CoV-2-Reactive Mucosal B Cells in the Upper Respiratory Tract of Uninfected Individuals.

Yanling LiuPatrick BudylowskiShilan DongZhijie LiSofiya GoroshkoLeslie Y T LeungEyal GrunebaumPaolo CampisiEvan Jon PropstNikolas E WolterJames M RiniAmin ZiaMario A OstrowskiGötz R A Ehrhardt
Published in: Journal of immunology (Baltimore, Md. : 1950) (2021)
SARS-CoV-2 is a respiratory pathogen that can cause severe disease in at-risk populations but results in asymptomatic infections or a mild course of disease in the majority of cases. We report the identification of SARS-CoV-2-reactive B cells in human tonsillar tissue obtained from children who were negative for coronavirus disease 2019 prior to the pandemic and the generation of mAbs recognizing the SARS-CoV-2 Spike protein from these B cells. These Abs showed reduced binding to Spike proteins of SARS-CoV-2 variants and did not recognize Spike proteins of endemic coronaviruses, but subsets reacted with commensal microbiota and exhibited SARS-CoV-2-neutralizing potential. Our study demonstrates pre-existing SARS-CoV-2-reactive Abs in various B cell populations in the upper respiratory tract lymphoid tissue that may lead to the rapid engagement of the pathogen and contribute to prevent manifestations of symptomatic or severe disease.
Keyphrases
  • sars cov
  • respiratory tract
  • respiratory syndrome coronavirus
  • coronavirus disease
  • endothelial cells
  • gene expression
  • early onset
  • dna methylation
  • drug induced
  • quantum dots