Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells.
Pengfei WangMohammad Aminur RahmanZhixiang ZhaoKristin WeissChao ZhangZhengjia ChenSelwyn J HurwitzZhuo G ChenDong M ShinYonggang KePublished in: Journal of the American Chemical Society (2018)
DNA origami is a promising molecular delivery system for a variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cancer cell lines remains elusive. We investigated the cellular uptake of four DNA-origami nanostructures (DONs) with programmed sizes and shapes in multiple human cancer cell lines. The cellular uptake efficiency of DONs was influenced by size, shape, and cell line. Scavenger receptors were responsible for the internalization of DONs into cancer cells. We observed distinct stages of the internalization process of a gold nanoparticle (AuNP)-tagged rod-shape DON, using high-resolution transmission electron microscopy. This study provides detailed understanding of cellular uptake and intracellular trafficking of DONs in cancer cells, and offers new insights for future optimization of DON-based drug delivery systems for cancer treatment.
Keyphrases
- circulating tumor
- single molecule
- cell free
- electron microscopy
- papillary thyroid
- high resolution
- cancer therapy
- nucleic acid
- squamous cell
- drug delivery
- atomic force microscopy
- circulating tumor cells
- young adults
- current status
- reactive oxygen species
- childhood cancer
- lymph node metastasis
- smoking cessation
- liquid chromatography
- induced pluripotent stem cells