A high-throughput system used to determine frequency and distribution of phosphine resistance across large geographical regions.
David I SchlipaliusAndrew G TuckHervoika PavicGregory J DaglishManoj K NayakPaul R EbertPublished in: Pest management science (2018)
The type of structure in which the grain is stored had a strong effect on resistance allele frequency. We observed higher frequencies of resistance alleles in bunker storages at central sites compared with other storage types. This contributed to the higher frequencies of resistance alleles in bulk-handling facilities relative to farms. The discovery of a storage structure that predisposes insects to resistance highlights the utility of our high-throughput assay system for improvement of phosphine resistance management practices. © 2018 Society of Chemical Industry.