Plasma-Derived Nanoclusters for Site-Specific Multimodality Photo/Magnetic Thrombus Theranostics.

Traditional thrombolytic therapeutics for vascular blockage are affected by their limited penetration into thrombi, associated off-target side effects, and low bioavailability, leading to insufficient thrombolytic efficacy. We hypothesized that these limitations could be overcome by the precisely controlled and targeted delivery of thrombolytic therapeutics. A theranostic platform was developed that is biocompatible, fluorescent, magnetic, and well-characterized, with multiple targeting modes. This multimodal theranostic system can be remotely visualized and magnetically guided toward thrombi, non-invasively irradiated by near-infrared (NIR) phototherapies, and remotely activated by actuated magnets for additional mechanical therapy. Magnetic guidance can also improve the penetration of nanomedicines into thrombi. In a mouse model of thrombosis, the thrombosis residues were reduced by ca. 80% and with no risk of side effects or of secondary embolization. This strategy not only enabled the progression of thrombolysis but also accelerated the lysis rate, thereby facilitating its prospective use in time-critical thrombolytic treatment. This article is protected by copyright. All rights reserved.