MtCLE08 , MtCLE16, and MtCLE18 Transcription Patterns and Their Possible Functions in the Embryogenic Calli of Medicago truncatula .
Andrei A KudriashovNatalia S ZlydnevaElena P EfremovaVarvara E TvorogovaLudmila A LutovaPublished in: Plants (Basel, Switzerland) (2023)
CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. CLE genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of WOX genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the MtWOX9-1 gene, stimulating somatic embryogenesis in Medicago truncatula . Three CLE peptides were found which could probably be such regulators, but none of them was found to influence MtWOX9-1 expression in the embryogenic calli. Nevertheless, overexpression of one of CLE genes under study, MtCLE16 , decreased somatic embryogenesis intensity. Additionally, overexpression of MtCLE08 was found to suppress expression of MtWOX13a , a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation.
Keyphrases
- genome wide identification
- transcription factor
- poor prognosis
- copy number
- genome wide
- dna binding
- stem cells
- cell proliferation
- binding protein
- gene expression
- metabolic syndrome
- dna methylation
- type diabetes
- genome wide analysis
- polycystic ovary syndrome
- amino acid
- cell wall
- living cells
- pregnancy outcomes
- insulin resistance