Deinococcus taeanensis sp. nov., a Radiation-Resistant Bacterium Isolated from a Coastal Dune.

A Gram-stain-negative, nonspore-forming, nonmotile, aerobic, rod-shaped, and very pale orange-colored bacterial strain, designated TS293 T , was isolated from a sand sample obtained from a coastal dune after exposure to 3kGy of gamma (γ)-radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Deinococcus and clustered with D. deserti VCD115 T . The genome of strain TS293 T was 4.62 Mbp long (68.2% G + C content and 4124 predicted genes) divided into a 2.86Mb main chromosome and five plasmids. Many genes considered to be important to the γ-radiation and oxidative stress resistance of Deinococcus were conserved in TS293 T , but genome features that could differentiate TS293 T from D. deserti and D. radiodurans, the type species of the Deinococcus genus, were also detected. Strain TS293 T showed resistance to γ-radiation with D 10 values (i.e., the dose required to reduce the bacterial population by tenfold) of 3.1kGy. The predominant fatty acids of strain TS293 T were summed feature 3 (C 16:1 ω6c and/or C 16:1 ω7c) and iso-C 16:0 . The major polar lipids were two unidentified phosphoglycolipids and one unidentified glycolipid. The main respiratory quinone was menaquinone-8. Based on the phylogenetic, genomic, physiological, and chemotaxonomic characteristics, strain TS293 T represents a novel species, for which the name Deinococcus taeanensis sp. nov. is proposed. The type strain is TS293 T (= KCTC 43191 T  = JCM 34027 T ).