Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research.
Leonardo Perez de SouzaSaleh AlseekhFederico ScossaAlisdair Robert FerniePublished in: Nature methods (2021)
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC-HRMS-based metabolomics, with a focus on expanding metabolome coverage.
Keyphrases
- high resolution mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- gas chromatography
- simultaneous determination
- ms ms
- copy number
- loop mediated isothermal amplification
- label free
- affordable care act
- real time pcr
- solid phase extraction
- high resolution
- health insurance