Maternal obesity is associated with a sex-specific epigenetic programming in human neonatal monocytes.
Fabián Vega-TapiaRocio ArtigasCherie HernándezRicardo UauyPaola CasanelloBernardo J KrauseJose A Castro-RodriguezPublished in: Epigenomics (2020)
Aim: To determine changes in global DNA methylation in monocytes from neonates of women with obesity, as markers of an immune programming resulting from maternal obesity. Materials & methods: Cord blood monocytes were obtained from neonates born to women with obesity and normal weight, genome-wide differentially methylated CpGs were determined using an Infinium MethylationEPIC-BeadChip (850K). Results: No clustering of samples according to maternal BMI was observed, but sex-specific analysis revealed 71,728 differentially methylated CpGs in female neonates from women with obesity (p < 0.01). DAVID analysis showed increased methylation levels within genes involved in the innate immune response and inflammation. Conclusion: Maternal obesity induces, in a sex-specific manner, an epigenetic programming of monocytes that could contribute to disease later in life. Clinical trial registry: This study is registered in ClinicalTrials.gov NCT02903134.
Keyphrases
- dna methylation
- weight gain
- weight loss
- insulin resistance
- metabolic syndrome
- birth weight
- genome wide
- type diabetes
- immune response
- high fat diet induced
- cord blood
- clinical trial
- dendritic cells
- body mass index
- pregnancy outcomes
- low birth weight
- oxidative stress
- adipose tissue
- endothelial cells
- peripheral blood
- randomized controlled trial
- single cell
- pregnant women
- skeletal muscle
- study protocol
- copy number
- body weight