A video protocol for rapid dissection of mouse dorsal root ganglia from defined spinal levels.
James N SleighSteven J WestGiampietro SchiavoPublished in: BMC research notes (2020)
By following this method, the reader will be able to swiftly and accurately isolate specific lumbar, thoracic, and cervical DRG from mice. Dissected ganglia can then be used for RNA/protein analyses, subjected to immunohistochemical examination, and cultured as explants or dissociated primary neurons, for in-depth investigations of sensory neuron biology.