Expression of Long Noncoding RNAs in Fibroblasts from Mucopolysaccharidosis Patients.
Zuzanna CyskeLidia GaffkeKarolina PierzynowskaGrzegorz WegrzynPublished in: Genes (2023)
In this report, changes in the levels of various long non-coding RNAs (lncRNAs) were demonstrated for the first time in fibroblasts derived from patients suffering from 11 types/subtypes of mucopolysaccharidosis (MPS). Some kinds of lncRNA ( SNHG5 , LINC01705 , LINC00856 , CYTOR , MEG3 , and GAS5 ) were present at especially elevated levels (an over six-fold change relative to the control cells) in several types of MPS. Some potential target genes for these lncRNAs were identified, and correlations between changed levels of specific lncRNAs and modulations in the abundance of mRNA transcripts of these genes ( HNRNPC, FXR1 , TP53 , TARDBP , and MATR3 ) were found. Interestingly, the affected genes code for proteins involved in various regulatory processes, especially gene expression control through interactions with DNA or RNA regions. In conclusion, the results presented in this report suggest that changes in the levels of lncRNAs can considerably influence the pathomechanism of MPS through the dysregulation of the expression of certain genes, especially those involved in the control of the activities of other genes.
Keyphrases
- long non coding rna
- poor prognosis
- genome wide identification
- genome wide analysis
- gene expression
- end stage renal disease
- genome wide
- newly diagnosed
- chronic kidney disease
- ejection fraction
- transcription factor
- bioinformatics analysis
- prognostic factors
- induced apoptosis
- long noncoding rna
- dna methylation
- network analysis
- patient reported outcomes
- oxidative stress
- cell death
- signaling pathway
- extracellular matrix
- functional connectivity
- room temperature
- patient reported
- pi k akt