Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells.
Xiaotong WangMengyuan QuZili LiYuting LongKai HongHong-Gang LiPublished in: Stem cell research & therapy (2021)
VPA robustly promoted the differentiation of human pluripotent stem cells into SSCLCs, which involved the upregulated genes in Wnt signaling pathway and epigenetic changes. hiPSCs from NOA patients showed decreased SSCLC induction efficiency and Wnt signaling pathway gene expression, suggesting that SSC depletion in azoospermia testes might be associated with inactivation of Wnt signaling pathway. Our developed SSCLC induction protocol provides a reliable tool and model to study human germ cell development and male infertility.
Keyphrases
- newly diagnosed
- pluripotent stem cells
- signaling pathway
- stem cells
- gene expression
- endothelial cells
- cell proliferation
- pi k akt
- epithelial mesenchymal transition
- germ cell
- dna methylation
- induced pluripotent stem cells
- chronic kidney disease
- insulin resistance
- end stage renal disease
- adipose tissue
- metabolic syndrome
- peritoneal dialysis
- ejection fraction
- genome wide identification