Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells.
Mayra Colunga-SaucedoEdson Iván Rubio-HernándezMiguel A Coronado-IpiñaSergio Rosales-MendozaClaudia G CastilloMauricio Comas-GarcíaPublished in: Viruses (2022)
The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.
Keyphrases
- escherichia coli
- genome wide
- klebsiella pneumoniae
- crispr cas
- regulatory t cells
- dendritic cells
- genome wide identification
- zika virus
- induced apoptosis
- copy number
- poor prognosis
- aedes aegypti
- dengue virus
- nucleic acid
- dna methylation
- cell cycle arrest
- transcription factor
- hiv infected
- cell death
- cell proliferation
- disease virus
- endoplasmic reticulum stress
- binding protein
- oxidative stress
- subarachnoid hemorrhage