The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila -colonizing Orbaceae .
Katherine M ElstonLaila E PhillipsSean P LeonardEleanor YoungJo-Anne C HolleyTasneem AhsanullahBraydin McReynoldsNancy A MoranJeffrey E BarrickPublished in: bioRxiv : the preprint server for biology (2023)
in the wild but have not been included in laboratory studies examining how the gut microbiome affects fly nutrition, health, and longevity. Our work establishes a path for genetic studies aimed at understanding and altering interactions between these and other newly isolated bacteria and their hosts.