Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria.
Jianwei ChenXiqian JiangChengwei ZhangKevin R MacKenzieFabio StossiTimothy G PalzkillMeng C WangJin WangPublished in: ACS sensors (2017)
We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.
Keyphrases
- fluorescent probe
- living cells
- oxidative stress
- fluorescence imaging
- flow cytometry
- induced apoptosis
- cell death
- endoplasmic reticulum
- reactive oxygen species
- cell cycle arrest
- photodynamic therapy
- dna damage
- ischemia reperfusion injury
- high resolution
- diabetic rats
- endoplasmic reticulum stress
- cell proliferation
- pi k akt