Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells.
A Sales ConniffG EncaladaS PatelM BhandaryF Al-TakrouriLoree C HellerPublished in: RNA biology (2022)
Germline-encoded pattern recognition receptors [PRRs] in mammalian cells function in the detection of molecular patterns associated with pathogen invasion or cellular damage. A PRR subset is activated by the atypical presence and location of double-stranded RNA [dsRNA] or its synthetic analogue polyinosinic-polycytidylic acid [poly(I:C)], triggering pro-inflammatory signalling and death in many cell types. Poly(I:C) has been tested as a sole or combination cancer therapy in preclinical studies and clinical trials. The purpose of this study was to evaluate the effects of poly(I:C) transfection via electroporation on cell lines from a cancer of epithelial origin, 4T1 mammary carcinoma, and a cancer of mesenchymal origin, WEHI 164 fibrosarcoma. The effects of the poly(I:C) delivery on cell metabolism implicate the induction of cell death. A pro-inflammatory response was demonstrated by mRNA upregulation and the secretion of Type I interferon and several cytokines and chemokines. The mRNAs of dsRNA sensor DExD/H-box helicase 58/retinoic acid-inducible gene I protein [Ddx58/RIG-I] and sensor/co-sensor DEAH-box helicase 9 [Dhx9] were not regulated, but the mRNAs of RNA sensors toll-like receptor 3 [TLR3], interferon-induced with helicase C domain 1/melanoma differentiation-associated protein 5 [Ifih1/MDA5] and Z-DNA binding protein 1 [Zbp1] and co-sensors DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 [Ddx60] and interferon-inducible protein 204 [Ifi204] were upregulated in both cell lines. The mRNAs encoding signalling pathways components were present or upregulated in both cell types. These data demonstrate that RNA sensing effects can be amplified by electroporation delivery, potentially expanding the practicality of this immunotherapeutic approach.
Keyphrases
- binding protein
- toll like receptor
- inflammatory response
- cell death
- cell therapy
- single cell
- clinical trial
- transcription factor
- cancer therapy
- cell cycle arrest
- papillary thyroid
- nuclear factor
- dendritic cells
- stem cells
- immune response
- lps induced
- oxidative stress
- nucleic acid
- induced apoptosis
- squamous cell carcinoma
- lipopolysaccharide induced
- bone marrow
- cell proliferation
- signaling pathway
- deep learning
- circulating tumor
- single molecule
- dna repair
- genome wide analysis
- cell free
- long non coding rna
- quantum dots
- mesenchymal stem cells
- poor prognosis
- label free
- candida albicans
- genome wide
- endothelial cells
- study protocol
- protein protein