Towards Aldehydomics: Untargeted Trapping and Analysis of Reactive Diet-Related Carbonyl Compounds Formed in the Intestinal Lumen.
Sylvie ChevolleauMaria-Helena Noguer-MeirelesLoic MervantJean-François MartinIsabelle JouaninFabrice PierreNathalie NaudFrançoise GuéraudLaurent DebrauwerPublished in: Antioxidants (Basel, Switzerland) (2021)
Lipid peroxidation and subsequent formation of toxic aldehydes, such as 4-hydroxynonenal, is known to be involved in numerous pathophysiological processes, possibly including the development of colorectal cancer. This work aimed at the development of an untargeted approach using high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-HRMS) for tracking aldehydes in both suspect screening and untargeted methods in fecal water, representing the aqueous environment of colon epithelial cells. This original approach is based on the introduction of a characteristic isotopic labeling by selective derivatization of the carbonyl function using a brominated reagent. Following a metabolomics workflow, the developed methodology was applied to the characterization of aldehyde compounds formed by lipid peroxidation in rats fed two different diets differentially prone to lipoperoxidation. Derivatized aldehydes were first selectively detected on the basis of their isotopic pattern, then annotated and finally identified by tandem mass spectrometry. This original approach allowed us to evidence the occurrence of expected aldehydes according to their fatty acid precursors in the diet, and to characterize other aldehydes differentiating the different diets.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- high performance liquid chromatography
- mass spectrometry
- ultra high performance liquid chromatography
- gas chromatography
- simultaneous determination
- fatty acid
- solid phase extraction
- weight loss
- high resolution
- physical activity
- liquid chromatography tandem mass spectrometry
- gas chromatography mass spectrometry
- ms ms
- risk assessment
- magnetic resonance imaging
- computed tomography