A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer.

Paola CampomenosiElisabetta GiniDouglas M NoonanAlbino PoliPaola D'AntonaNicola RotoloLorenzo DominioniAndrea Imperatori

Published in: BMC biotechnology (2016)

Analyses of serum miRNAs performed with qPCR and ddPCR were largely concordant. Both qPCR and ddPCR can reliably be used to quantify circulating miRNAs, however, ddPCR revealed similar or greater precision and higher throughput of analysis.

Keyphrases

endothelial cells
single cell
induced pluripotent stem cells
high throughput
high resolution
real time pcr
pluripotent stem cells