Inhibitory Effects of Loganin on Adipogenesis In Vitro and In Vivo.
Hyoju JeonChang-Gun LeeHyesoo JeongSeong-Hoon YunJeonghyun KimLaxmi Prasad UpretyKang-Il OhShivani SinghJisu YooEunkuk ParkSeon-Yong JeongPublished in: International journal of molecular sciences (2023)
Obesity is characterized by the excessive accumulation of mature adipocytes that store surplus energy in the form of lipids. In this study, we investigated the inhibitory effects of loganin on adipogenesis in mouse preadipocyte 3T3-L1 cells and primary cultured adipose-derived stem cells (ADSCs) in vitro and in mice with ovariectomy (OVX)- and high-fat diet (HFD)-induced obesity in vivo. For an in vitro study, loganin was co-incubated during adipogenesis in both 3T3-L1 cells and ADSCs, lipid droplets were evaluated by oil red O staining, and adipogenesis-related factors were assessed by qRT-PCR. For in vivo studies, mouse models of OVX- and HFD-induced obesity were orally administered with loganin, body weight was measured, and hepatic steatosis and development of excessive fat were evaluated by histological analysis. Loganin treatment reduced adipocyte differentiation by accumulating lipid droplets through the downregulation of adipogenesis-related factors, including peroxisome proliferator-activated receptor γ ( Pparg ), CCAAT/enhancer-binding protein α ( Cebpa ), perilipin 2 ( Plin2 ), fatty acid synthase ( Fasn ), and sterol regulatory element binding transcription protein 1 ( Srebp1 ). Loganin administration prevented weight gain in mouse models of obesity induced by OVX and HFD. Further, loganin inhibited metabolic abnormalities, such as hepatic steatosis and adipocyte enlargement, and increased the serum levels of leptin and insulin in both OVX- and HFD-induced obesity models. These results suggest that loganin is a potential candidate for preventing and treating obesity.
Keyphrases
- high fat diet induced
- insulin resistance
- high fat diet
- weight gain
- adipose tissue
- fatty acid
- metabolic syndrome
- skeletal muscle
- binding protein
- type diabetes
- body mass index
- weight loss
- birth weight
- body weight
- induced apoptosis
- high glucose
- transcription factor
- mouse model
- endothelial cells
- drug induced
- diabetic rats
- signaling pathway
- cell proliferation
- cell death
- oxidative stress
- preterm birth
- human health
- replacement therapy
- atomic force microscopy