Analysis of Vitreous Samples by the Cellient® Automated Cell Block System: A Six-year Review of Specimens in a Uveitis Population.
Tine JacobsDietmar Rudolf ThalBirgit WeynandJoachim Van CalsterRita Van GinderdeurenPublished in: Ocular immunology and inflammation (2020)
Purpose: To further evaluate the value of a standardized method to analyze vitreous samples using the Cellient® automated cell block system in the diagnosis of unsolved uveitis.Methods: Six hundred sixty-four pure vitreous samples obtained from patients with unsolved uveitis between March 2012 and May 2018 at University Hospitals Leuven, fixed in PreservCyt® and processed by the Cellient tissue processor, were included in the study.Results: In 86.3% of the cases, sufficient material was found for diagnosis. A diagnosis of acute inflammation was made in 20.2% of the cases. In 34.0% of the cases, the diagnosis was chronic active inflammation; in 26.5%, low-grade inflammation; and in 5.6%, a malignant process.Conclusion: Our standardized protocol can be used to diagnose endophthalmitis, lymphoma, granulomatous disease, inactive and storage disease, and metastatic infiltration. Cellient analysis of vitreous specimens had a diagnostic yield of 86.3% in unsolved uveitis cases.
Keyphrases
- juvenile idiopathic arthritis
- low grade
- oxidative stress
- ankylosing spondylitis
- single cell
- machine learning
- randomized controlled trial
- small cell lung cancer
- high grade
- high throughput
- cell therapy
- deep learning
- squamous cell carcinoma
- liver failure
- stem cells
- rheumatoid arthritis
- intensive care unit
- mesenchymal stem cells
- drug induced
- respiratory failure
- hepatitis b virus
- mechanical ventilation
- fine needle aspiration
- idiopathic pulmonary fibrosis