OsSLI1, a homeodomain containing transcription activator, involves abscisic acid related stress response in rice (Oryza sativa L.).
Xi HuangMin DuanJiakai LiaoXi YuanHui ChenJiejie FengJi HuangHong-Sheng ZhangPublished in: TheScientificWorldJournal (2014)
Homeodomain-leucine zipper type I (HD-Zip I) proteins are involved in the regulation of plant development and response to environmental stresses. In this study, OsSLI1 (Oryza sativa stress largely induced 1), encoding a member of the HD-Zip I subfamily, was isolated from rice. The expression of OsSLI1 was dramatically induced by multiple abiotic stresses and exogenous abscisic acid (ABA). In silico sequence analysis discovered several cis-acting elements including multiple ABREs (ABA-responsive element binding factors) in the upstream promoter region of OsSLI1. The OsSLI1-GFP fusion protein was localized in the nucleus of rice protoplast cells and the transcriptional activity of OsSLI1 was confirmed by the yeast hybrid system. Further, it was found that OsSLI1 expression was enhanced in an ABI5-Like1 (ABL1) deficiency rice mutant abl1 under stress conditions, suggesting that ABL1 probably negatively regulates OsSLI1 gene expression. Moreover, it was found that OsSLI1 was regulated in panicle development. Taken together, OsSLI1 may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
Keyphrases
- transcription factor
- gene expression
- dna binding
- dna methylation
- genome wide identification
- tyrosine kinase
- poor prognosis
- binding protein
- induced apoptosis
- stress induced
- arabidopsis thaliana
- nuclear factor
- immune response
- drug delivery
- inflammatory response
- smoking cessation
- signaling pathway
- amino acid
- molecular dynamics simulations
- cell cycle arrest
- endoplasmic reticulum stress
- heat shock protein
- pi k akt